Living protocols for genome editing

The field of genome editing is moving at breakneck speed and protocols are rapidly evolving. We’ve already made a few different posts on tips, tricks, and protocols for genome editing and regulation. But effectively sharing protocols and making sure that they’re up to date is a daunting task. Much better to have a community-driven effort, where a starter protocol can be tweaked and updated as new developments come along.

That’s why I’m happy to share that we’ve recently started putting our methods on Protocols.io. This is an open repository for protocols, which the great feature of “forking”. This means you can start from a protocol that you like, tweak it as desired, make a record of the tweaks, and re-publish your changes. Everything is also linkable to a DOI, which means you can potentially reference online protocols from within papers.

IGI protocols for T7E1 assays, in vitro transcription of guide RNAs, Cas9 RNP nucleofection, and more are available at https://www.protocols.io/g/innovative-genomics-initiative/protocols. 

Here’s an explanatory video, from the protocols.io team.