We have deposited many of the lab’s detailed protocols on Protocols.IO at http://www.protocols.io/groups/cornlab.
Some of the most popular protocols are
- Cas9 expression and purification
- In vitro transcription of guide RNAs
- Nucleofection of Cas9 RNP for high efficiency genome editing
- T7 Endonuclease (T7E1) assay
- Reamplification of CRISPR libraries
|Example T7E1 digestion (treated vs untreated) for thirteen genes that were targeted with the Cas9 RNP. These results are representative for a larger set of 200 genes targeted in this experiment.|