Protocols & Reagents

Protocols

We have deposited many of the lab’s detailed protocols on Protocols.IO at http://www.protocols.io/groups/igi.

Some of the most popular protocols are

t7e1_example
Example T7E1 digestion (treated vs untreated) for thirteen genes that were targeted with the Cas9 RNP. These results are representative for a larger set of 200 genes targeted in this experiment.

Reagents and Cell Lines

We make most of the lab’s published (and some unpublished) reagents available on Addgene at https://www.addgene.org/Jacob_Corn/. Email us if you read about a reagent in one of our papers and can’t find it on Addgene (e.g. HDR donor oligos, in vitro transcribed sgRNAs).

All of our CRISPR stable cell lines (e.g. for genome-wide screening) are available through the Berkeley Cell Culture facility. Below are a few examples of CRISPRi/a lines we have deposited through the facility. Check the link above for an up-to-date list of lines.

  • Jurkat dCas9-VPR (CRISPRa)
  • Jurkat dCas9-KRAB (CRISPRi)
  • HCT116 dCas9-KRAB (CRISPRi)
  • 3T3-L1 dCas9-KRAB (CRISPRi)

Contact Allison Killilea at ankillilea@berkeley.edu for more information on Corn lab CRISPR lines. Cell lines described in papers but not distributed by the Berkeley Cell Culture facility are available by direct request. Contact our lab manager Julia Chu at juliachu@berkeley.edu to request a cell line.

Contact Us

Questions and/or comments about Corn Lab and its activities may be addressed to:

JACOB.CORN@BIOL.ETHZ.CH

Share: