Publications

Atlastins remodel the endoplasmic reticulum for selective autophagy.
Liang JR, Lingeman E, Ahmed S, Corn JE.
J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185.

CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.
Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schäfer AJ, Floor SN, Corn JE.
Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27.

In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathway. 
Wienert B, Shin J, Zelin E, Pestal K, Corn JE.
PLoS Biol. 2018 Jul 16;16(7):e2005840. doi: 10.1371/journal.pbio.2005840. eCollection 2018 Jul.

Sometimes you’re the scooper, and sometimes you get scooped: How to turn both into something good.
Kim JS, Corn JE.
PLoS Biol. 2018 Jul 16;16(7):e2006843. doi: 10.1371/journal.pbio.2006843. eCollection 2018 Jul.

Enhanced genome editing with Cas9 ribonucleoprotein in diverse cells and organisms.
Farboud B, Jarvis E, Roth TL, Shin J, Corn JE, Marson A, Meyer BJ, Patel NH, and Hochstrasser ML.
Journal of Visualized Experiments (JoVE), ISSUE 135, 5/25/2018, DOI: 10.3791/57350

BARD1 is necessary for ubiquitylation of nucleosomal histone H2A and for transcriptional regulation of estrogen metabolism genes.
Mikaela D. Stewart, Elena Zelin, Abhinav Dhall, Tom Walsh, Esha Upadhyay, Jacob E. Corn, Champak Chatterjee, Mary-Claire King and Rachel E. Klevit.
Proc Natl Acad Sci U S A, 2018 Feb 6;115(6):1316-1321.

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homologous directed DNA repair.
Lee K, Conboy M, Park HM, Jiang FG, Kim HJ, Dewitt M A, Mackley VA, Chang K, Rao A, Skinner C, Shobha T, Liu H, Huang WC, Lan F, Bray NL, Li S, Corn JE, Kataoka K, Doudna JA, Conboy I, Murthy N.
Nature Biomedical Engineering, DOI: 10.1038/s41551-017-0137-2 [Epub ahead of print] (2017)

E Pluribus Unum (“Out of Many, One”): CRISPR Modeling of Myeloid Expansion.
Shin J, Corn JE.
Cell Stem Cell, Oct 5;21(4):415-416. (2017)

Production of Purified CasRNPs for Efficacious Genome Editing.
Lingeman E, Jeans C, Corn JE.
Curr Protoc Mol Biol., Oct 2;120:31.10.1-31.10.19. (2017)

Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements.
Mumbach MR, Satpathy AT, Boyle EA, Dai C, Gowen BG, Cho SW, Nguyen ML, Rubin AJ, Granja JM, Kazane KR, Wei Y, Nguyen T, Greenside PG, Corces MR, Tycko J, Simeonov DR, Suliman N, Li R, Xu J, Flynn RA, Kundaje A, Khavari PA, Marson A, Corn JE, Quertermous T, Greenleaf WJ, Chang HY.
Nature Genetics, Sep 25. doi: 10.1038/ng.3963. [Epub ahead of print] (2017)

Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Dimitre R. Simeonov, Benjamin G. Gowen, Mandy Boontanrart, Theodore L. Roth, John D. Gagnon, Maxwell R. Mumbach, Ansuman T. Satpathy, Youjin Lee, Nicolas L. Bray, Alice Y. Chan, Dmytro S. Lituiev, Michelle L. Nguyen, Rachel E. Gate, Meena Subramaniam, Zhongmei Li, Jonathan M. Woo, Therese Mitros, Graham J. Ray, Gemma L. Curie, Nicki Naddaf, Julia S. Chu, Hong Ma, Eric Boyer, Frederic Van Gool, Hailiang Huang, Ruize Liu, Victoria R. Tobin, Kathrin Schumann, Mark J. Daly, Kyle K. Farh, K. Mark Ansel, Chun J. Ye, William J. Greenleaf, Mark S. Anderson, Jeffrey A. Bluestone, Howard Y. Chang, Jacob E. Corn & Alexander Marson
Nature, Sep 7;549(7670):111-115. (2017)

Protein engineering to increase the potential of a therapeutic antibody Fab for long-acting delivery to the eye.
Tesar D, Luoma J, Wyatt EA, Shi C, Shatz W, Hass PE, Mathieu M, Yi L, Corn JE, Maass KF, Wang K, Dion MZ, Andersen N, Loyet KM, van Lookeren Campagne M, Rajagopal K, Dickmann L, Scheer JM, Kelley RF.
mAbs, Aug 30:1-9. (2017)

Disabling Cas9 by an anti-CRISPR DNA mimic.
Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA.
Sci Adv., Jul 12;3(7):e1701620. (2017)

Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering.
Lee K, Mackley VA, Rao A, Chong AT, DeWitt MA, Corn JE, Murthy N.
Elife, May 2;6. pii: e25312. (2017)

Flexibility and Design: Conformational Heterogeneity along the Evolutionary Trajectory of a Redesigned Ubiquitin.
Biel JT, Thompson MC, Cunningham CN, Corn JE, Fraser JS.
Structure, Apr 12. pii: S0969-2126(17)30069-2. (2017)

Genome editing via delivery of Cas9 ribonucleoprotein.
DeWitt MA, Corn JE, Carroll D.
Methods, May 15;121-122:9-15. (2017) 

Cornerstones of CRISPR-Cas in drug discovery and therapy.
Christof Fellmann, Benjamin G. Gowen, Pei-Chun Lin, Jennifer A. Doudna and Jacob E. Corn
Nat Rev Drug Discov., Feb;16(2):89-100. (2017)

Efficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells.
Michael Dillona, Yiyuan Yina Jianhui Zhoua, Luke McCartyb, Diego Ellermanb, Dionysos Slagac, Teemu T. Junttilac, Guanghui Hand, Wendy Sandovald, Meric A. Ovacike, Kedan Line, Zhilan Huf, Amy Shenf, Jacob E. Corn, Christoph Spiessa and Paul J. Carter
mAbs., Feb/Mar;9(2):213-230. (2017)

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.
DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D*, Martin DI*, Corn JE*.
Science Translational Medicine
, 2016 Oct 12;8(360):360ra134. (2016)
*co-corresponding 

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, and Weissman JS.
Elife, Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. (2016)

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes.  
Richardson CD, Ray GJ, Bray NL, and Corn JE
Nat Commun., Aug 17;7:12463. (2016)

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Richardson CD, Ray GJ, DeWitt MA, Curie GL, and Corn JE
Nat Biotechnol., Mar;34(3):339-44 (2016)

Using Protein Motion to Read, Write, and Erase Ubiquitin Signals.
Phillips AH, Corn JE
J Biol Chem, 290(44):26437-44. (2015)

Co-opting CRISPR to deliver functional RNAs.
Lin PC, Corn JE
Nat Methods, 12(7):613-4. (2015)

Biotechnology. A prudent path forward for genomic engineering and germline gene modification.
Baltimore D, Berg P, Botchan M, Carroll D, Charo RA, Church G, Corn JE, Daley GQ, Doudna JA, Fenner M, Greely HT, Jinek M, Martin GS, Penhoet E, Puck J, Sternberg SH, Weissman JS, Yamamoto KR
Science, 348(6230):36-8. (2015)

USP30 and Parkin homestatically regulate ubiquitin chain abundance on mitochondria.
Cunningham CN, Baughman JM, Phu L, Tea J, Yu C, Coons M, Kirkpatrick DS, Bingol B and Corn JE
Nature Cell Biology, 17:160-9. (2015) 

Ubiquitin in inflammation: the right linkage makes all the difference. 

Corn JE and Vucic D

Nature Structural and Molecular Biology, 21:297-300. (2014) 

Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor.

Mukund S, Shang Y, Clarke HJ, Madjidi A, Corn JE, Kates L, Kolumam G, Luis E, Murray J, Zhang Y, Hötzel I, Koth CM, and Allan BB

Journal of Biological Chemistry, 288:36168-71. (2013)

The RosettaCon 2012 special collection: code writ on water, documentation writ in stone.
André I and Corn J
PLoS One, 8:e73775. (2013)

Decoding a chain letter for degradation.
Cunningham CN, Corn JE
Structure, 21:1068-70. (2013)

Conformational dynamics control ubiquitin-deubiquitinase interactions and influence in vivo signaling.

Phillips AH, Zhang Y, Cunningham CN, Zhou L, Forrest WF, Liu PS, Steffek M, Lee J, Tam C, Helgason E, Murray JM, Kirkpatrick DS, Fairbrother WJ, Corn JE
PNAS, 110:11379-84. (2013)

Conformational stabilization of ubiquitin yields potent and selective inhibitors of USP7.
Zhang Y, Zhou L, Rouge L, Phillips AH, Lam C, Liu P, Sandoval W, Helgason E, Murray J, Wertz IE, Corn JE 

Nature Chemical Biology, 9:51-8. (2013)

USP30 inhibitors and methods of use. Patent application WO2014041111 A1.
Bingol B, Corn J, Zhang Y 

Filed 9/13/2014, priority date 9/17/2012. Patent pending.

Binding mechanism of metal-NTP substrates and stringent-response alarmones to bacterial DnaG- type primases.
Rymer RU, Solorio FA, Tehranchi AK, Chu C, Corn JE, Keck JL, Wang JD, Berger JM

Structure, 20:1478-89. (2012)

Engineered conformationally-stabilized proteins.
Corn JE, Zhang Y, Phillips A

Patent application US20130281314 A1, filed 3/14/2013, priority date 3/16/2012. Patent pending.

Community-wide assessment of protein-interface modeling suggests improvements to design methology.
Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T, Bernauer J, Poupon A, Azé J, Soner S, Ovalı SK, Ozbek P, Tal NB, Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Pérez-Cano L, Pons C, Fernández-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L, Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, Tropsha A, Esquivel-Rodríguez J, Kihara D, Stranges PB, Jacak R, Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D

J Mol Biol, 414:289-302. (2011)

Hotspot-centric de novo design of protein binders.
Fleishman SJ, Corn JE, Strauch EM, Whitehead TA, Karanicolas J, Baker D

J Mol Biol, 413:1047-62. (2011)

Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complex.

Bentley ML, Corn JE, Dong KC, Phung Q, Cheung TK, Cochran AG
EMBO J, 30:3285-97. (2011)

RosettaScripts: a scripting language interface to the Rosetta macromolecular modeling suite.
Fleishman SJ, Leaver-Fay A, Corn JE, Strauch EM, Khare SD, Koga N, Ashworth J, Murphy P, Richter F, Lemmon G, Meiler J, Baker D 

PLoS One, 6:e20161. (2011)

Computational design of proteins targeting the conserved stem region of influenza hemagglutinin.
Fleishman SJ, Whitehead TA, Ekiert DC, Dreyfus C, Corn JE, Strauch E-M, Wilson IA, and Baker D

Science, 332:816-21. (2011)

A de novo protein binding pair by computational design and directed evolution.
Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Chung S, Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione G, Spiegel C, Liu D, and Baker D
Molecular Cell, 42:250-60. (2011)

Molecular determinants of origin discrimination by Orc1 initiators in archaea.
Dueber EC, Costa A, Corn JE, Bell SD, Berger JM 

Nucleic Acids Research, 39:3621-31. (2011)

Rosetta3: An object-oriented software suite for the simulation and design of macromolecules.
Leaver-Fay A, Tyka M, Lewis SM, Lange OF, Thompson J, Jacak R, Kaufman K, Renfrew PD, Smith CA, Sheffler W, Davis IW, Cooper S, Treuille A, Mandell DJ, Richter F, Ban YE, Fleishman SJ, Corn JE, Kim DE, Lyskov S, Berrondo M, Mentzer S, Popović Z, Havranek JJ, Karanicolas J, Das R, Meiler J, Kortemme T, Gray JJ, Kuhlman B, Baker D, Bradley P

Methods in Enzymology, 487:545-74. (2011)

Rosetta in CAPRI rounds 13-19.
Fleishman SJ, Corn JE, Strauch EM, Whitehead TA, Andre I, Thompson J, Havranek JJ, Das R, Bradley P, Baker D

Proteins, 78(15):3212-8. (2010)


Automated electron-density sampling reveals widespread conformational polymorphism in proteins.
Lang PT, Ng HL, Fraser JS, Corn JE, Echols N, Sales M, Holton JM, Alber T
Protein Science, 19(7):1420-31. (2010)

THAP proteins target specific DNA sites through bipartite recognition of adjacent major and minor grooves.

Sabogal A, Lyubimov AY, Corn JE, Berger JM, Rio DC
Nature Structural and Molecular Biology, 17(1):117-23. (2010)

Bioactive TGF-beta can associate with lipoproteins and is enriched in those containing apolipoprotein E3.

Tesseur I, Zhang H, Brecht W, Corn J, Gong JS, Yanagisawa K, Michikawa M, Weisgraber K, Huang Y, Wyss-Coray T
Journal of Neurochemistry, 110(4):1254-62. (2009)

Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic C. tepidum: comparison with thermophilic and mesophilic homologs.
Ratcliff K, Corn J, Marqusee S 

Biochemistry, 48(25):5890-8. (2009)

Hyperthermophilic Aquifex aeolicus initiates primer synthesis on a limited set of trinucleotides comprised of cytosines and guanines.
Larson MA, Bressani R, Sayood K, Corn JE, Berger JM, Griep MA, Hinrichs SH

Nucleic Acids Research, 36(16):5260-9. (2008)

Identification of a DNA primase template tracking site redefines primer synthesis geometry.
Corn JE, Pelton JG, Berger JM 

Nature Structural and Molecular Biology, 15(2):163-9. (2008)

Replication origin recognition and deformation by a heterodimeric archaeal Cdc6/Orc1 complex.
Dueber EL, Corn JE, Bell SD, Berger JM 

Science, 317(5842):1210-3. (2007)

FASTDXL: A generalized screen to trap disulfide stabilized complexes for use in structural studies.
Corn JE, Berger JM 

Structure, 15(7):773-80. (2007)

Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions.
Corn JE, Berger JM

Nucleic Acids Research, 34(15):4082-8. (2006)

Crosstalk between primase subunits can act to regulate primer synthesis in trans.
Corn JE, Pease PJ, Hura GL, Berger JM

Molecular Cell, 20(3):391-401. (2005)

Contact Us

Questions and/or comments about Corn Lab and its activities may be addressed to:

JACOB.CORN@BIOL.ETHZ.CH

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