Congratulations to Mandy!

Mandy just received her PhD in Molecular and Cellular Biology from UC Berkeley in August. Her dissertation “Flipping the Hemoglobin Switch and Discovering Regulators Involved in Fetal Hemoglobin Reactivation” can be found here:
Author(s): Boontanrart, Mandy | Advisor(s): Corn, Jacob E | Abstract: The fetal to adult hemoglobin switch is a developmental process by which fetal hemoglobin becomes silenced after birth and replaced by adult hemoglobin. Diseases caused by defective or missing adult hemoglobin, such as Sickle Cell Disease or β-Thalassemia, can be ameliorated by reactivating fetal hemoglobin. We discovered that knockdown or knockout of β-globin, a subunit of adult hemoglobin, led to robust upregulation of γ-globin, a subunit of fetal hemoglobin. This phenomenon suggested that red blood cells have an inherent ability to upregulate fetal hemoglobin in the event that adult hemoglobin is lacking.We developed multiple gene-editing tools in an immortalized erythroid cell model to investigate the molecular mechanisms behind the increase in fetal hemoglobin. Time-course transcriptomics identified ATF4, a transcription factor, as a causal regulator of this response. Further analysis also converged upon downregulation of MYB and BCL11A, known repressors of γ-globin, described in detail in chapter 2. Further work in chapter 3 explores other possible fetal hemoglobin regulators as discovered by CRISPRi arrayed mediated knockdown experiments. This work furthers our understanding of fundamental mechanisms of gene regulation and how cellular and molecular events influence red blood cell differentiation.


Your email address will not be published. Required fields are marked *


Latest News

September 8, 2021

Congratulations to Mandy!

Congratulations to Mandy and team for winning the audience vote at the ETH 3Pi Pitch Competition, where ETH Pioneer Fellows pitched their projects to an audience...

September 1, 2021

Lab retreat in Zermatt

Our lab retreat for 2021 was held in Zermatt, in view of the Matterhorn. We spent the first day doing extemporaneous speed talks (3 minutes maximum!) and discussing...

June 17, 2021

Congratulations to Eric and John on the EMBO fellowships!

We are excited to share the news that Eric Aird and John Fielden were both awarded EMBO long-term fellowships, which support excellent postdoctoral researchers...

News Archive