Next generation guide RNAs for genome-wide CRISPR screens

IGI Co-Director Jonathan Weissman, Scientific Director Jacob Corn, Research Scientist Chong Park, and other colleagues have developed the next generation of guide RNAs (gRNAs) for high-throughput CRISPR screens. Establishing rules for the design of effective gRNAs is critical to making CRISPR activation and repression screens more specific and robust. Based on recent observations that compacted DNA inhibits Cas9 access, the team modified their guide RNA design algorithm to target more open, expanded regions of human and mouse genomes. Taking chromatin, position, and sequence features into account allowed for the design of highly active guide RNA libraries.

gRNA library design tools are available on Github.  The improved gRNA libraries will soon be available on AddGene.

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation  
eLife | Jonathan Weissman, Jacob Corn, Chong Park, et al | September 23, 2016

SUBMIT A COMMENT

Your email address will not be published. Required fields are marked *

Filters

Latest News

November 20, 2020

Welcome to Eric

Eric joined the Corn lab as a Postdoc in November 2020. He has received his PhD in Biochemistry, Molecular Biology, and Biophysics from the University of Minnesota...

October 15, 2020

Welcome to Roman

Roman joined the Corn lab in October 2020. Earlier this year he has received his Ph.D. from the University of Boulder, where he had developed tools by combining...

October 7, 2020

Welcome to Susanne

Susanne joined the Corn Lab in September 2020 to support the team with High Throughput sequencing applications. She received her PhD in Paleogenomics at the...

News Archive