Next generation guide RNAs for genome-wide CRISPR screens

IGI Co-Director Jonathan Weissman, Scientific Director Jacob Corn, Research Scientist Chong Park, and other colleagues have developed the next generation of guide RNAs (gRNAs) for high-throughput CRISPR screens. Establishing rules for the design of effective gRNAs is critical to making CRISPR activation and repression screens more specific and robust. Based on recent observations that compacted DNA inhibits Cas9 access, the team modified their guide RNA design algorithm to target more open, expanded regions of human and mouse genomes. Taking chromatin, position, and sequence features into account allowed for the design of highly active guide RNA libraries.

gRNA library design tools are available on Github.  The improved gRNA libraries will soon be available on AddGene.

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation  
eLife | Jonathan Weissman, Jacob Corn, Chong Park, et al | September 23, 2016

SUBMIT A COMMENT

Your email address will not be published. Required fields are marked *

Filters

Latest News

September 8, 2021

Congratulations to Mandy!

Congratulations to Mandy and team for winning the audience vote at the ETH 3Pi Pitch Competition, where ETH Pioneer Fellows pitched their projects to an audience...

September 1, 2021

Lab retreat in Zermatt

Our lab retreat for 2021 was held in Zermatt, in view of the Matterhorn. We spent the first day doing extemporaneous speed talks (3 minutes maximum!) and discussing...

June 17, 2021

Congratulations to Eric and John on the EMBO fellowships!

We are excited to share the news that Eric Aird and John Fielden were both awarded EMBO long-term fellowships, which support excellent postdoctoral researchers...

News Archive

Tweets