Lab News

NSF Graduate Fellow Emily Lingeman et al. have published a chapter “Production of Purified CasRNPs for Efficacious Genome Editing” in Current Protocols in Molecular Biology. This article describes how to make a Cas9 RNP and outlines its use for gene editing in human cells. Check it out if you’d like to get started with RNP-based gene editing.

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IGI Scientific Director of Biomedicine Jacob Corn has received the NIH Director’s New Innovator Award that provides $1.5 million over five years to pursue high-risk, high-reward work that could have implications for human health. The National Institutes of Health’s (NIH) High-Risk, High-Reward Research program, part of the NIH Common Fund, funded 86 awards to exceptionally creative scientists proposing to use highly innovative approaches to tackle major challenges in biomedical research.

Corn lab will use CRISPR-Cas9 gene editing to explore how cells recycle damaged organs, such as mitochondria. Dysfunction in organelle autophagy has been implicated in diverse diseases, including neurodegeneration, lysosomal storage disorders and cancer.  Corn lab will use next- generation CRISPR-Cas9 genome editing and regulation technologies, combined with cellular biochemistry and imaging to discover the pathways that signal for the remodeling and lysosomal degradation of multiple organelles.  Our work will reveal the mechanisms by which cells maintain organelle homeostasis and respond to organelle damage or stress, which could suggest new strategies to treat diseases associated with improper organelle autophagy.

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The work of IGI scientists “Discovery of stimulation-responsive immune enhancers with CRISPR activation” was recently published in Nature. The researchers used CRISPR activation to find DNA “switches” that control key genes in the immune response and autoimmune disease. Post-doc Benjamin Gowen and PhD student Dimitre Simeonov were the lead authors. IGI Scientific Director Jacob Corn and IGI affiliate Alexander Marson jointly supervised the work. Important contributions were made by several Corn Lab members and alumni, including Mandy Boontanrart, Nicolas Bray, Therese Mitros, Jordan Ray, Gemma Curie, Nicki Naddaf, Julia Chu, and Hong Ma.  A summary of the research was featured by the UCSF News Center.

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A series of interviews and lab visits by The Daily Californian show what it’s like to work inside some of UC Berkeley’s most high-level labs.  Our lab is featured in the article “The Genome Revolution: Inside UC Berkeley’s CRISPR labs” .  

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We’d like to welcome Beeke Wienert to join our lab as a postdoctoral researcher.  She received her PhD in Molecular Genetics and Biochemistry from UNSW Sydney in 2016 and then continued to work as a postdoctoral researcher investigating molecular mechanisms of hemoglobin disorders with Merlin Crossley.  Her main research interests are transcription factors, their binding profiles and how genomic variation causes disease and she is currently using CRISPR/Cas9 to study transcriptional programs of red blood cell enucleation.

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Congratulations to our undergraduate researcher, Sharon Feng!  She will receive the Summer Undergraduate Research Fellowships— Rose Hills Experience  to continue her research during summer.

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